diff --git a/esphome/components/rp2/__init__.py b/esphome/components/rp2/__init__.py
index 76dd240f7a..d46858d397 100644
--- a/esphome/components/rp2/__init__.py
+++ b/esphome/components/rp2/__init__.py
@@ -195,6 +195,8 @@ def _parse_platform_version(value: Any) -> str:
 #  * The new version needs to be thoroughly validated before changing the
 #    recommended version as otherwise a bunch of devices could be bricked
 #  * For all constants below, update platformio.ini (in this repo)
+#  * rp2_pio pins its own pioasm build (PIOASM_REPO_VERSION in rp2_pio/__init__.py); check
+#    https://github.com/earlephilhower/pico-quick-toolchain/releases for a matching release
 
 # The default/recommended arduino framework version
 #  - https://github.com/earlephilhower/arduino-pico/releases
diff --git a/esphome/components/rp2_pio/__init__.py b/esphome/components/rp2_pio/__init__.py
index 1b2236ae8e..bfdff910da 100644
--- a/esphome/components/rp2_pio/__init__.py
+++ b/esphome/components/rp2_pio/__init__.py
@@ -8,9 +8,9 @@ DEPENDENCIES = ["rp2"]
 DOMAIN = "rp2_pio"
 
 
-PIOASM_REPO_VERSION = "1.5.0-b"
+PIOASM_REPO_VERSION = "4.1.0"
 PIOASM_REPO_BASE = f"https://github.com/earlephilhower/pico-quick-toolchain/releases/download/{PIOASM_REPO_VERSION}"
-PIOASM_VERSION = "pioasm-2e6142b.230216"
+PIOASM_VERSION = "pioasm-9fdfe11.250530"
 PIOASM_DOWNLOADS = {
     "linux": {
         "aarch64": f"aarch64-linux-gnu.{PIOASM_VERSION}.tar.gz",
@@ -21,8 +21,8 @@ PIOASM_DOWNLOADS = {
         "amd64": f"x86_64-w64-mingw32.{PIOASM_VERSION}.zip",
     },
     "darwin": {
-        "x86_64": f"x86_64-apple-darwin14.{PIOASM_VERSION}.tar.gz",
-        "arm64": f"x86_64-apple-darwin14.{PIOASM_VERSION}.tar.gz",
+        "x86_64": f"x86_64-apple-darwin20.4.{PIOASM_VERSION}.tar.gz",
+        "arm64": f"aarch64-apple-darwin20.4.{PIOASM_VERSION}.tar.gz",
     },
 }
 
diff --git a/tests/components/rp2040_pio_led_strip/test.rp2350-ard.yaml b/tests/components/rp2040_pio_led_strip/test.rp2350-ard.yaml
new file mode 100644
index 0000000000..25135f1d39
--- /dev/null
+++ b/tests/components/rp2040_pio_led_strip/test.rp2350-ard.yaml
@@ -0,0 +1,2 @@
+packages:
+  rp2040_pio_led_strip: !include common.yaml